a549 dualtm ko rig  (InvivoGen)

Journal: iScience

Article Title: Targeted delivery of immune-stimulating bispecific RNA, inducing apoptosis and anti-tumor immunity in cancer cells

doi: 10.1016/j.isci.2024.109068

Figure Lengend Snippet: Simultaneous activation of RIG-I and silencing of PLK1 mediated by mAb-siPLK1-5′ppp 1 on A549-Dual siPLK1-5′ppp was delivered by either RNAiMAX or EphA2-antibody. (A) RIG-I-dependent activation of the IRF pathway (N = 6, triplicates). (B) RIG-I-dependent activation of the NF-κB pathway (N = 6, triplicates). Dual activity of the siPLK1-5′ppp delivered by either RNAiMAX or EphA2-antibody regarding RIG-I/PLK1 expression levels or cell-cycle arrest. (C) Western-blot on RIG-I, PLK1, and GAPDH, showing a simultaneous PLK1 knockdown and RIG-I activation, 72 h after incubation at 40 nM. (D) Proportion of cells in G2/M phase analyzed by FACS after 24 h of incubation at 40 nM (N = 2, triplicates). Data are presented as mean ± SD. Significancy was assessed by comparing treated cells with non-treated cells, ns = non-significant, ∗∗∗ = p < 0.0005, ∗∗∗∗ = p < 0.00005).

Article Snippet: A549-Dual RIG-I KO , InvivoGen , a549d-korigi.

Techniques: Activation Assay, Activity Assay, Expressing, Western Blot, Knockdown, Incubation

Journal: iScience

Article Title: Targeted delivery of immune-stimulating bispecific RNA, inducing apoptosis and anti-tumor immunity in cancer cells

doi: 10.1016/j.isci.2024.109068

Figure Lengend Snippet: mAb-siPLK1-5′ppp induces A549-Dual cell death by apoptosis and activates PBMCs to promote anti-tumor inflammation A549-Dual confluence over time after treatments with transfected siRNAs (A) or ARCs (B for A549 cells alone, C for a mixture of A549/PBMC: 1/5). One picture of A549 cells was taken every 2 h for 4 days, PBMC were excluded by the software because of the difference of size between A549 and PBMC, N = 2 with two different donors of PBMCs—(D) Cytokine quantification after mAb-siRNA-5′ppp 1 treatment for 24 h at 100 nM to the A549/PBMC mixture. Data are presented as mean ± SD. Significancy was assessed by comparing the secreted cytokine obtained from mAb-siRNA-5′ppp 1 compared to the non-treated mixture of cells (ns = non-significant, ∗ = p < 0.05, ∗∗ = p < 0.005, ∗∗∗ = p < 0.0005, ∗∗∗∗ = p < 0.00005), N = 1, triplicates.

Article Snippet: A549-Dual RIG-I KO , InvivoGen , a549d-korigi.

Techniques: Transfection, Software

Journal: iScience

Article Title: Targeted delivery of immune-stimulating bispecific RNA, inducing apoptosis and anti-tumor immunity in cancer cells

doi: 10.1016/j.isci.2024.109068

Figure Lengend Snippet:

Article Snippet: A549-Dual RIG-I KO , InvivoGen , a549d-korigi.

Techniques: Isolation, Recombinant, WST-1 Assay, Control, Software